Conas ta tu Niall?
Im going to help with this whole focus thing, autofocus, depth of field, focal points.
Read this, fill in you camera, lens, aperture and distance details and you will figure it out: http://www.dofmaster.com/dofjs.html
So theres a couple of things to know and it removes confusion. After youve done the above homework assignment only...
First is, you can let the camera chose exactly where to focus (instant death with close up photography), or you can take control of exactly what you want it to focus on. By default, the camera has multiple FOCUS points that flash when you have focus. Its telling you it has something within the points that flashed focused, so you can take the shot. But that poor Bee is out on its own there, - ignored by the camera FOCUS points. When you take control, you make the camera use only ONE FOCUS point, and you place that focus point on Mr Bee, and it will FOCUS on it. So the Bee will be in focus, and then become the FOCAL point of your shot. Its very easy to select a single focus point, - check the manual, very simple.
The amount of the flower/plant in front of the Bees Bum and behind the bees head that will also appear in focus, is simply called the depth of field. That link will each you that the smaller the aperture (larger the number, as aperture is a fraction, 1/aperture).
The unique issue that a close shot brings, is that ideally you need a macro lens to allow very close focusing, - youre is not a macro; and the closer you are to the subject, the aperture and depth if field rules remain the same, assuming you are focused on the bee. BUT you shutter speed must be VERY fast. Think of looking through binoculars, - the slightest movement is magnified to a blur. Thats macro.
So it looks to me as helpfully pointed out in the attached mod, that the camera, - youre friend,(?) focused in front of the Bee; and the Bee was moving, as they do.
So, f/8, shutter speed 1/2000, and ISO at around 1600. Sharp Bee and a bit of noise.