An attractive and colourful subject.
Looking at your EXIF data I would suggest a couple of things here.
Firstly are you shooting handheld or on a tripod? For this type of close focus work where depth of field is minimal it is advisable to use a solid support for your camera such as a tripod. This minimises the risk of camera shake and moving in and out of focus as handheld, and even with a steady hand, the subtle movements of the body can be enough to miss focus as the shutter is pressed. It allows an improved range of exposure combinations without upping the iSO to high levels that could introduce other issues in image quality such as noise. You can focus on the right aperture for the depth of field rather than be compromised by trying to maintain a high enough shutter speed to avoid the risk of camera shake.
Here the centre of the flower is nice and sharp however the outer edge of the petals are out of focus and create a halo of softness around this central area. This is a little distracting and would make a stronger image if the petals from top down to the centre were sharp. You can always soften the background further in editing software if the background sharpness pulls in enough to become a distraction. In all I would have gone for a much smaller aperture here such as f16 to get the required depth of field. If you shot handheld you could have afforded to come up in ISO to say 400 to allow 1/100s at f16 without compromising quality.
I would also say the lighting is quite strong and you are starting to lose some of the petal textural detail as the strong yellow starts to bleed. Coming down slightly in exposure or saturation at the processing stage may pull back a little more of this detail.
My final point would be about the composition. It feels unbalanced and the strong highlights on the background leaves are a distraction. I would suggest a tighter crop to really focus in on the flowerhead which is where all the beauty in this image lies.
I hope that helps and I will upload a mod to demonstrate what I mean.